Table 1.
List of antibodies utilized and tissue biopsy treatment conditions for the immunohistochemical biomarker evaluation conducted at baseline and at week 12
| Manufacture | Clone name |
H202 block |
Non-specific protein block |
1st antibody concentration and incubation time |
2nd antibody polymer and incubation time |
|
|---|---|---|---|---|---|---|
| VEGF | Santa Cruz Bio Tech (Santa Cruz, CA, USA) |
A-20, RXH |
22’ | Dako Serum Free Protein Block for 7’ |
1:200,65’ | DAKO LSAB+Streptavidin for 15’, HPR for 15’ |
| Cyclin D1 | Lab Vision (Fremont, CA, USA) |
SP-4, RXH |
15’ | 10% FBS for 35’ |
1:100,65’ | DAKO Envision Link+ for 30’ |
| Ki-67 | DAKO (Carpinteria, CA, USA) |
MIB- 1, MXH |
15’ | 10% FBS for 35’ |
1:200,65’ | DAKO Envision Link+ for 30’ |
| P53 | DAKO (Carpinteria, CA, USA) |
Do-7, MXH |
15’ | 10% FBS for 35’ |
1:400,65’ | DAKO Envision Link+ for 30’ |