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. 2014 Nov 19;12:317. doi: 10.1186/s12967-014-0317-8

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© Beloki et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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PM staining before and after the adherence process. Plastic adherence method was applied to PBMCs from G-CSF mobilized donors (n = 11) and PM+ cells were quantified in unmanipulated PBMCs and the non-adherent cell fraction. Specific (CD3+CD8+PM+) and non-specific (CD8-PM+) PM binding was analyzed. Percentages were analyzed from CD45+ cell gate.