Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 18;14:297. doi: 10.1186/s12870-014-0297-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Siena et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

N69 partial cDNA derives from a plant-specific gene encoding a trimethyl guanosine synthase. (A) A consensus cDNA sequence was assembled using N69 cDNA, a N69-extended fragment obtained by RACE (black bars) and reads from RNA 454 sequencing in sexual (C4-4x) and apomictic (Q4117) reproductive tissues (white bars). This sequence contains a 2004 bp predicted ORF coding for a product sharing homology with yeast TRIMETHYL GUANOSINE SYNTHASE 1 (TGS1). (B) Primers were designed in order to amplify 4 different sequence fragments (F1, F2, F3, F4), which were amplified and sequenced for validation, from reproductive tissues of apomictic (Q4117) and sexual (C4-4x) genotypes. (C) Phylogenetic analysis of proteins similar to yeast TGS1 from several yeast and plant model species revealed that the Paspalum notatum predicted product is highly similar with plant specific members of the TGS1 protein family defined by the association of the RNA-cap guanine-N2 methyltransferase domain with a WW domain. Domains are indicated as colored ticks. ClustalW alignments are shown on the right (arrows indicate Pasalum notatum sequences).