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. 2014 May 22;25(12):2764–2777. doi: 10.1681/ASN.2012080823

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Copyright © 2014 by the American Society of Nephrology

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Figure 3. — Lmx1b and FoxC are both required for nphs2 expression in zebrafish. (A) MO-mediated knockdown of lmx1b and foxc1 genes. Microscopic images of 4-dpf morphants of individual or double knockdown as well as controls are displayed. Pericardial edema is indicated (arrow) in bright-field imaging, and glomerular GFP expression is indicated (arrowhead) in dark-field imaging. The percentage of pericardial edema is shown. Number of morphants is indicated in parentheses. (B) Combinatorial knockdown of lmx1b.1 and foxc1a genes. A low dose of lmx1b.1-MO (0.25 ng) or foxc1a-MO (1 ng) was individually injected in Tg(podocin:GFP) embryos. Glomerular GFP expression is indicated (arrowhead) in dark-field imaging. For double knockdown, 0.25 ng lmx1b.1-MO and 1 ng foxc1a-MO were coinjected in embryos. The percentage of pericardial edema in 4-dpf morphants is shown. Number of morphants is indicated in parentheses.