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. 2014 Nov 18;14:586. doi: 10.1186/s12879-014-0586-0

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© Bartels et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Conventional 40-cycle Helicobacter cinaedi PCR 1 and 2 targeting the cdt gene resulting in 659-bp and 359-bp amplicons, respectively. Polyacrylamid-based gel electrophoresis of PCR products using Agilent 2200 Tape Station (Agilent Technologies). L, molecular weight ladder; lane 1 and 6, 400 ng DNA of aortic valve (formalin-fixed); lane 2 and 7, 80 ng DNA of aortic valve (formalin-fixed); lane 3 and 8, 250 ng DNA of aortic valve (fresh); lane 4 and 9, 50 ng DNA of aortic valve (fresh); lane 5 and 10, negative control (5 μl of elution buffer).