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. 2014 Nov 21;7:521. doi: 10.1186/s13071-014-0521-7

Table 1.

PCR parameters for amplification of DNA of target organisms

Target organism Forward primer (5’-3’) No. of cycles Annealing temperature (°C) Primer concentration (pmol) Product size (bp) Reference
Reverse primer (5’-3’)
Anaplasma sp. Ehr.u.for: GTT TGA TCC TGG CTC AGG AYD AAC 30 66.8 12.5 619 [15]
ERB2rev: CTC TTT CGA CCT CTA GTC TAG C
Piroplasms (nested) 1st 40 68 25 561 [16]
BTH-1 F: CCT GAG AAA CGG CTA CCA CAT CT
BTH-1R: TTG CGA CCA TAC TCC CCC CA
2nd
GF2: GTC TTG TAA TTG GAA TGA TGG 40 60 50
GR2: CCA AAG ACT TTG ATT TCT CTC
Ehrlichia canis Ehr.u.for: GTT TGA TCC TGG CTC AGG AYD AAC 30 65.0 20 619 [15]
Ehr.CCE.rev: CTC TTT CGA CCT CTA GTC TAG C
Hepatozoon canis HEPF: ATA CAT GAG CAA AAT CTC AAC 35 57.0 10 660 [17]
HEPR: CTT ATT ATT CCA TGC TGC AG
Rickettsia sp. ITS-F: GAT AGG TCG GGT GTG GAA G 35 52 1 342 – 533 [18]
ITS-R: TCG GGA TGG GAT CGT GTG