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Primers and probe used in this study
| Region | Primers and probe | Position | Sequence | Product length |
|---|---|---|---|---|
| ORF 3 | Forward primer1 (HEV.Fa) | 5278-5294 | GTGCCGGCGGTGGTTTC | 81 bp |
| Forward primer2 (HEV.Fb) | 5278-5296 | GTGCCGGCGGTGGTTTCTG | ||
| Reverse primer (HEV.R) | 5340-5359 | GCGAAGGGGTTGGTTGGATG | ||
| Probe (HEV.P) | 5300-5320 | FAM-TGACMGGGTTGATTCTCAGCC-BHQ1 |
ORF = open reading frame.
