Table 3.
Target genes and primer pairs used in gene expression assays.
| Target Gene | Gene Function | Primer Pair (5ʹ-3ʹ) | Annealing Temperature (°C) |
Product Size (bp) | Reference |
|---|---|---|---|---|---|
| 16S rDNA | Housekeeping | F-TGGAGCATGTGGTTTAATTCGA R-TGCGGGACTTAACCCAACA |
60 | 159 | [32] |
| glt | Species specific, coding for glutamate synthase | F-AATCTTTGTCGGTACACGATATTCTTCACG R-CGTAATGAGATTTCAGTAGATAATACAACA |
58 | 108 | [33] |
| nuc | Virulence factor, coding for thermonuclease | F-GACTATTATTGGTTGATCCACCTG R- GCCTTGACGAACTAAAGCTTCG |
60 | 218 | [34] |
| mecA | Methicillin resistance | F-TCCAGATTACAACTTCACCAGG R-CCACTTCATATCTTGTAACG |
57 | 162 | [35] |