Temperature |
37°C |
Well cured for 1.5% collagen |
4°C |
Difficult to solidify |
Room temperature |
Difficult to solidify |
pH |
pH 5–6 |
Difficult to solidify, large pore size (~200 μm) |
pH 6–7 |
Weakly cured, medium pore size (~120 μm) |
pH 7–8 |
Well cured, small pore size (~80 μm) |
Collagen stock concentration |
1.0% (w/v) |
Gelation takes a long time (~48 hours), relatively large pore size (∼200 μm) |
1.5% (w/v) |
Relatively large pore size (~200 μm) |
2.0% (w/v) |
Nonhomogenous gelation, relatively small pore size (~100 μm) |
2.5% (w/v) |
Nonhomogeneous gelation, relatively small pore size (~100 μm) |
Curing duration |
~30 minutes |
Less cured for 1.5% collagen |
~60 minutes |
Well cured for 1.5% collagen |
~90 minutes |
Well cured for 1.5% collagen, susceptible to drying of hydrogel |