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. Author manuscript; available in PMC: 2015 May 24.
Published in final edited form as: Nat Commun. 2014 Nov 24;5:5556. doi: 10.1038/ncomms6556

Figure 4. BLM-dependent unwinding activity on a G4 substrate.

Figure 4

(a) Quantification of BLM unwinding efficiency at 50 nM BLM and 2 mM ATP for the G4, G4 (5 nt) and G4 (10 nt) substrates (Error bar = S.E.M. n=5)

(b/c) FRET histograms of the G4 (5 nt) and G4 (10 nt) substrates (b and c respectively) in the presence of 50 nM BLM and at different ATP concentrations.

(d/e) Representative single molecule trajectories of the G4 (5 nt) and G4 (10 nt) substrates (d and e respectively) in the presence of 50 nM BLM and 10 μM ATP. FRET trajectories were fitted with HMM (Cyan).

(f/g) Generated TDP matrix of the G4 (5 nt) and G4 (10 nt) substrates (f and g, respectively) in the presence of 50 nM BLM and 10 μM ATP.

(h/i) The calculated mean unfolding and refolding rates for the G4 (5 nt) and G4 (10 nt) substrates (h and i respectively) in the presence of 50 nM BLM and at different ATP concentrations. (Error bars = S.E.M. n > 15 for all measurements).