(a) Quantification of BLM unwinding efficiency at 50 nM BLM and 2 mM ATP for the G4, G4 (5 nt) and G4 (10 nt) substrates (Error bar = S.E.M. n=5)
(b/c) FRET histograms of the G4 (5 nt) and G4 (10 nt) substrates (b and c respectively) in the presence of 50 nM BLM and at different ATP concentrations.
(d/e) Representative single molecule trajectories of the G4 (5 nt) and G4 (10 nt) substrates (d and e respectively) in the presence of 50 nM BLM and 10 μM ATP. FRET trajectories were fitted with HMM (Cyan).
(f/g) Generated TDP matrix of the G4 (5 nt) and G4 (10 nt) substrates (f and g, respectively) in the presence of 50 nM BLM and 10 μM ATP.
(h/i) The calculated mean unfolding and refolding rates for the G4 (5 nt) and G4 (10 nt) substrates (h and i respectively) in the presence of 50 nM BLM and at different ATP concentrations. (Error bars = S.E.M. n > 15 for all measurements).