FIGURE 1.

Anti-promastigote activity of Artemisia annua leaves (AALEO). Log phase Leishmania donovani promastigotes were incubated with AALEO (100 μg ml^-1) for 7 days at 22°C as described in “Materials and Methods.” (A) Viable parasites enumerated microscopically every day (B) promastigote growth reversibility analysis after 4 days of culture, post AALEO withdrawal (C) morphological analysis of promastigotes by light microscopy (40×) at 72 h post-treatment and (D)% viability ascertained upon incubation of promastigotes for 72 h with increasing concentrations of AALEO (0–100 μg ml^-1) for IC₅₀ determination (inset). Each point or bar corresponds to the mean ± SE of triplicate samples and is representative of one of three independent experiments.