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Detection of apoptosis in L. donovani promastigotes by annexin V-FITC and PI co-staining. Parasites were incubated with AALEO or pentamidine (100 μg ml-1) for 72 h, and analyzed by flow cytometry as described in the Methods. (A) Dual parametric dot plots combining Annexin V-FITC and PI fluorescence, (B) % apoptotic cell population. This is a representative profile of three independent experiments.
