FIGURE 4.

Artemisia annua leaves treated promastigotes represent TdT-mediated dUTP nick-end labeling (TUNEL) positivity and dyskinetoplastidy. Exponential phase promastigotes were incubated with AALEO or pentamidine (100 μg ml^-1) for 72 h. Cells were fixed and stained with dUTP–FITC in the presence of TdT. (A) Bar graphs representing MFI of TUNEL expression as analyzed by flow cytometry (B) Image acquisition via a fluorescence microscope under 10× objective. (C) Treated and untreated parasites were probed with PI for assessment of dyskinetoplastidy and images taken under 20× objective as described in Section “Materials and Methods.” N and K represent nuclear and kinetoplast DNA, respectively. Each bar corresponds to the mean ± SE of triplicate samples and is a representative profile of three independent experiments. Images are representative of one of three similar results.