FIGURE 5.

Artemisia annua leaves induces cell cycle arrest in L. donovani promastigotes at sub-G₀–G₁ phase. After treatment with AALEO and pentamidine (100 μg ml^-1) for 72 h, the promastigotes were fixed in chilled methanol, probed with PI and the cells acquired using a BD LSR-II flow cytometer for subsequent analysis using Cell Quest software as described in Section “Materials and Methods.” Inset shows the cell cycle distribution. This is the representative profile of three independent experiments.