FIGURE 7.

Antileishmanial efficacy of AALEO on established L. donovani infection. Ten weeks infected BALB/c mice were left untreated (INF) or treated for 10 days with PBS (VC), AALEO (50, 100, or 200 mg/kg bw, daily, i.p.) or amphotericin B (AMB; 5 mg/kg bw, alternate days, i.v.) as elaborated in Section “Materials and Methods.” Ten days post-treatment (A) splenic and hepatic parasite burden (LDU) were determined by stamp smear method, (B) morphology of spleen, and (C) weights of liver and spleen were also determined. The dotted lines indicate the respective organ weights of naïve mice. Data represent the mean ± SE for five animals per group. Data were tested by ANOVA. Differences between means were assessed for statistical significance by Tukey’s test (**P≤ 0.01; ***P≤ 0.001). Results are from one of three representative experiments.