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Schematic representation of the T-DNA region of pCAMBIA 1380 binary plasmid used for transformation of deembryonated cotyledons with Agrobacterium tumefaciens strain LBA 4404. The position of the primers used in PCR assays is shown by arrows on top of the mtlD gene. LB, left T-DNA border sequence; RB, right border sequence; 35S, CaMV35S promoter; and mtlD, mannitol-1-phosphate dehydrogenase.
