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. 2003 Jul;92(1):145–151. doi: 10.1093/aob/mcg116

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graphic file with name mcg116f2a.jpg — Fig. 2. Origin and development of root buds and somatic embryos in EMB‐9‐RAD plants of the interspecific hybrid H. annuus × H. tuberosus, as indicated by root and stem sections stained with haematoxylin. A and B, Bud primordia (arrows) at different stages of development flanking lateral roots (lr). C, Initial stage of a bud meristem (arrow) in the cortex adjacent to a lateral root. D, Meristematic dome (arrow). E, Root bud with leaf primordia (lp) and differentiating procambial strands (arrows). F, Root bud showing a double stratified tunica (arrow) and leaf primordia (lp). G, Cluster of abnormal morphogenetic structures. H, Longitudinal section of stem showing meristematic primordia (arrows) initiated at the cut edge. I, Magnification of H. Parenchyma cells after repeated divisions (arrows). J, Torpedo‐stage of a somatic embryo. K, Late globular‐stage of a somatic embryo (arrow). L and M, Abnormal embryo‐like structures. N, Late torpedo‐stage of a somatic embryo with procambial strands evident (arrowheads). Bars = 120 µm (A), 70 µm (B, C, E, F, J, M), 40 µm (D, K, L), 300 µm (G), 500 µm (H), 50 µm (I) and 200 µm (N).

graphic file with name mcg116f2b.jpg — Fig. 2. Origin and development of root buds and somatic embryos in EMB‐9‐RAD plants of the interspecific hybrid H. annuus × H. tuberosus, as indicated by root and stem sections stained with haematoxylin. A and B, Bud primordia (arrows) at different stages of development flanking lateral roots (lr). C, Initial stage of a bud meristem (arrow) in the cortex adjacent to a lateral root. D, Meristematic dome (arrow). E, Root bud with leaf primordia (lp) and differentiating procambial strands (arrows). F, Root bud showing a double stratified tunica (arrow) and leaf primordia (lp). G, Cluster of abnormal morphogenetic structures. H, Longitudinal section of stem showing meristematic primordia (arrows) initiated at the cut edge. I, Magnification of H. Parenchyma cells after repeated divisions (arrows). J, Torpedo‐stage of a somatic embryo. K, Late globular‐stage of a somatic embryo (arrow). L and M, Abnormal embryo‐like structures. N, Late torpedo‐stage of a somatic embryo with procambial strands evident (arrowheads). Bars = 120 µm (A), 70 µm (B, C, E, F, J, M), 40 µm (D, K, L), 300 µm (G), 500 µm (H), 50 µm (I) and 200 µm (N).