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. 2014 Nov 18;14:290. doi: 10.1186/s12870-014-0290-7

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© Wang et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Multiple sequence alignment and phylogenetic relationship for the SiLEA14 protein and its homologs. (A) Multiple sequence alignment of SiLEA14 with its homologs from various plant species. The conserved “LEA_2” motif (PF03168) is boxed. (B) Phylogenetic relationships of SiLEA14 and its homologs. The divergence of the clades between the monocots and dicots is highlighted. SiLEA14 and its two homologs in foxtail millet are highlighted by solid dark circle and triangle, respectively. The GenBank accession numbers are as follows: SiLEA14 (Setaria italic, KJ767551), AtLEA14 (Arabidopsis thaliana, NM_100029), Lea14-A (Zea mays, NM_001159174), D95-4 (Glycine max, U08108), IbLEA14 (Ipomoea batatas, GU369820), ER5 (Solanum lycopersicum, U77719), Lemmi9 (Solanum lycopersicum, Z46654), CaLEA6 (Capsicum annuum, AF168168), OsLEA5 (Oryza sativa, JF776156), pcC27-45 (Craterostigma plantagineum, M62990), pcLEA14 (Pyrus communis, AF386513), At1g01470 (Arabidopsis thaliana, BT015111), LEA14-A (Gossypium hirsutum, M88322), Lea14 homolog (Glycine max, NM_001251780), At2g46140 (Arabidopsis thaliana, NM_130176), Os01g0225600 (Oryza sativa, NM_001048996), LEA14-A-like (Brachypodium distachyon, XM_003567779), LOC100274480 (Zea mays, NM_001148839), SORBIDRAFT 09 g029860 (Sorghum bicolour, XM_002441543), LEA-like protein (Cenchrus americanus, AY823547), OsI21161 (Oryza sativa, CM000130), Os05g0526700 (Oryza sativa, NM_001062639), Os05g0584300 (Oryza sativa, NM_001062985), At2g44060 (Arabidopsis thaliana, BT024723), LOC100285131 (Zea mays, EU970969) and umc2111 (Zea mays, NM_001155750). The sequences used for alignment and phylogenetic tree construction were shown in Additional file 2.