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. 2014 Nov 18;14:290. doi: 10.1186/s12870-014-0290-7

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© Wang et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of GUS gene in pro SiLEA14 ::GUS transgenic Arabidopsis upon various stresses determined by qRT-PCR. One-week-old seedlings were treated with 100 μM ABA, 250 mM NaCl and 20% (m/v) PEG 6000 solution for 0, 3, 6 and 18 h, respectively. Total RNA was extracted from at least 30 whole seedlings per treatment. The expression of GUS in proSiLEA14::GUS transgenic Arabidopsis was determined by qRT-PCR using GUS-specific primers (Additional file 4). Actin2 was used to normalize the gene expression. Data represent means and standard errors for three biological replicates.