Table 3.
The effect of crude aqueous Piper betle L. extract (CA) on the activity of partially purified CAG (based on the rate of production of water insoluble glucan, determined from the glucose content). Results are expressed as the mean value ± standard deviation of 9 determinations. CHX - chlorhexidine. (Statistic analysis: one-way ANOVA)
| Experiments | Enzyme activity (μmol glucose/min) |
|---|---|
| Negative control (without the CA) | 0.034±0.002 |
| 0.5 mg mL-1 CA | 0.024±0.002a ,b |
| 1.0 mg mL-1 CA | 0.022±0.002a ,b |
| 1.5 mg mL-1 CA | 0.018±0.001a ,b |
| 2.0 mg mL-1 CA | 0.014±0.001a ,b |
| 2.5 mg mL-1 CA | 0.010±0.001a ,c |
| 4 mg mL-1 CA | nd |
| Positive control (0.12 % CHX) | 0.009±0.001a |
a
- p<0.0001 comparing plant extract-treated glucosyltransferase samples to negative control
b
- p<0.0001 comparing plant extract-treated glucosyltransferase samples to positive control
c
- p<0.0675 comparing CA 2.5 mg mL-1-treated glucosyltransferase samples to positive control
nd- not detectable