Figure 3.
List of antibodies used for immunohistochemical staining
| Antibody Specificit | Clone | Dilution |
|---|---|---|
| CK7* | OV-TL12/30 | 1:200 |
| CK8* | 35ßH11 | 1:150 |
| CK19** | B170 | 1:100 |
| CK20* | KS20.8 | 1:50 |
| Hmw CKs* | 34ßE12 | 1:200 |
| EMA* | E29 | 1:200 |
| CEA* | 11-7 | 1:200 |
| CEA* | Polyclonal | 1:400 |
| GFAP* | 6F2 | 1:100 |
| S-100* | COW S-100 | 1:1400 |
| SMA* | 1A4 | 1:200 |
| MSA* | HHF35 | 1:5000 |
| p63* | 4A4 | 1:150 |
| Vimentin* | V9 | 1:400 |
| Collagen IV* | CIV22 | 1:80 |
| p53* | DO-7 | 1:50 |
| Ki-67* | KI-55 | 1:100 |
CK (cytokeratin), Hmw (high-molecular-weight), EMA (epithelial membrane antigen), CEA (carcinoembryonic antigen), GFAP (glial fibrillary acidic protein), SMA (smooth muscle actin), MSA (muscle specific actin)
Source: * Dako, Carpinteria, CA, USA
**
Novocastra Laboratories Ltd, Newcastle, UK