Supplemental Table S2. Rotational relaxation time ρ (ns) of laurdan, M-laurdan, MoC-laurdan and C-laurdan at 20°C.

Anisotropy values (r) were converted into rotational relaxation time (ρ) taking into account the average lifetime (τ) of the probes using the following equation: $\rho =\frac{3\tau }{\left(\frac{r_0}{r}\right)-1}$, [G. Weber, Adv. Prot. Res. 1953, 8, 415], where r ₀ is the intrinsic anisotropy (set to the theoretical maximal value of 0.4 for calculations).

Sample		laurdan				M-laurdan
		r	τ (ns)	ρ (ns)		r	τ (ns)	ρ (ns)
DPPC		0.26	5.82	33.13		0.27	4.86	30.30
PSM		0.23	4.28	18.27		0.27	4.06	25.01
BSM		0.20	3.82	11.69		0.24	3.81	17.25
DPPC/chol (6:4)		0.25	5.41	25.87		0.26	4.45	25.70
PSM/chol (2:1)		0.24	5.34	22.96		0.26	4.36	23.68
BSM/chol (2:1)		0.23	4.61	18.27		0.24	4.29	20.20
POPC/chol (2:1)		0.21	4.35	14.01		0.22	3.98	14.71
DOPC/BSM/chol (1:1:1)		0.19	4.36	12.11		0.21	4.06	12.82
DOPC/PSM/chol (1:1:1)		0.19	4.30	11.41		0.23	3.85	15.05
DOPC/chol (2:1)		0.18	3.75	9.48		0.19	3.81	10.00
DOPC		0.18	3.04	7.40		0.18	3.33	8.01
POPC		0.16	3.19	6.09		0.17	3.16	6.81
Sample		MoC-laurdan				C-laurdan
		r	τ (ns)	ρ (ns)		r	τ (ns)	ρ (ns)
DPPC		0.22	5.24	20.07		0.26	5.02	29.23
PSM		0.15	2.77	4.98		0.27	2.87	17.45
BSM		0.11	2.00	2.35		0.22	2.96	10.70
DPPC/chol (6:4)		0.22	4.73	16.75		0.26	4.69	26.31
PSM/chol (2:1)		0.18	4.03	10.34		0.26	5.05	27.84
BSM/chol (2:1)		0.17	3.26	6.88		0.25	4.44	21.46
POPC/chol (2:1)		0.16	3.21	6.27		0.23	3.53	14.46
DOPC/BSM/chol (1:1:1)		0.14	3.17	5.05		0.22	3.36	12.79
DOPC/PSM/chol (1:1:1)		0.15	3.16	5.94		0.24	3.72	17.38
DOPC/chol (2:1)		0.14	3.15	4.88		0.22	2.62	9.67
DOPC		0.13	3.17	4.83		0.21	2.13	7.42
POPC		0.11	3.03	3.51		0.21	2.26	7.58