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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Stem Cell Res. 2014 Jul 27;13(2):275–283. doi: 10.1016/j.scr.2014.07.006

Figure 4.

Figure 4

Delivery of endocrine fate-specifying factors to expanded pancreatic organoid cultures yields insulin-expressing cells in vitro. (A) GFP expression in MIP-GFP mouse pancreatic organoid cells 72h after mock transduction or transduction with adenovirus expressing mouse Pdx1, Neurog3 and MafA, measured by flow cytometry. (B-D) Expression of endocrine- and duct-associated genes in Ad-PNM (Pdx1-NeuroG3-MafA) treated pancreatic organoid cells relative to mouse islet cDNA, measured by qRTPCR. Organoid cell cDNA was recovered from the entire culture (GFP+ and GFP-).