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. Author manuscript; available in PMC: 2014 Nov 25.
Published in final edited form as: Chem Biol. 2013 Sep 12;20(10):1245–1254. doi: 10.1016/j.chembiol.2013.07.017

Figure 3. In Vitro Effect of Inhibitory Compounds on CsgA Polymerization.

Figure 3

(A) ThT fluorescence of freshly purified 5 μM CsgA with or without 50 μM compound. Reduction in ThT fluorescence corresponds to inhibition of CsgA polymerization.

(B) CsgA solubility after overnight incubation with or without compound by SDS-PAGE. Compounds that inhibit CsgA polymerization kept CsgA in a soluble SDS-sensitive state.

(C) CsgA incubated overnight with compounds was spotted onto a nitrocellulose membrane and probed with a gammabody grafted with the CsgA R1 sequence that detects CsgA amyloid structure.

(D) Transmission electron microscopy of freshly purified CsgA incubated for 6 hr revealed abundant fibers, whereas no curli fibers were detected by when freshly purified CsgA was incubated for 6 hr with the inhibitor compound 7. Scale bars represent 0.5 μM.

See also Figure S1.