Figure 4.

Anti-inflammatory effects of CB₂ receptors in the frontal cortex; pro-inflammatory enzymes. (A) Western blot and densitometric analysis of NOS-2 in homogenates of the frontal cortex from untreated WT mice and WT mice treated with JWH-133, both with and without stress. In (B), results from CB2xP mice, with and without stress and in (C) for CB2-KO mice, with and without stress. Data are normalized by β-actin (lower band) and are representative of three experiments. *P < 0.05, **P < 0.01 versus CWT; ^#P < 0.05, ^###P < 0.001 versus SWT; two-way anova with Bonferroni post test. (D) Western blot and densitometric analysis of COX-2 in homogenates of the frontal cortex from untreated WT mice and WT mice treated with JWH-133, both with and without stress. In (E), results from CB2xP mice, with and without stress and in (F) for CB2-KO mice, with and without stress. Data are normalized by β-actin (lower band) and are representative of three experiments. *P < 0.05, **P < 0.01 versus CWT; ^##P < 0.01 versus SWT; two-way anova with B onferroni post test. (G) Levels of PGE₂ in homogenates of the frontal cortex from untreated WT mice and WT mice treated with JWH-133, both with and without stress. In (H), results from CB2xP mice, with and without stress and in (I) for CB2-KO mice, with and without stress. The data represent the mean ± SEM of six mice. *P < 0.05 versus CWT; ^#P < 0.05, ^###P < 0.001 versus SWT; two-way anova with Bonferroni post test. AU, arbitrary units.