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. 2014 May 15;171(11):2827–2841. doi: 10.1111/bph.12623

Figure 4.

Figure 4

Concentration-dependent activation of p38MAPK (A, C, E) and ERK1/2 (B, D, F) by H3 relaxin, H2 relaxin or R3(BΔ23–27)R/I5 in CHO-RXFP3 cells. H3 relaxin, H2 relaxin or R3(BΔ23–27)R/I5 all activated p38MAPK and ERK1/2 (5 min treatment) with H3 relaxin having the highest efficacy. The effect of R3(BΔ23–27)R/I5 on H3 relaxin-stimulated p38MAPK or ERK1/2 activation was tested either by co-addition of R3(BΔ23–27)R/I5 and H3 relaxin (C, D) or by pre-incubation of cells with R3(BΔ23–27)R/I5 for 1 h prior to H3 relaxin treatment (E, F). R3(BΔ23–27)R/I5 inhibited H3 relaxin-stimulated p38MAPK and ERK1/2 activation in a concentration-dependent manner under both experimental conditions. Data are mean ± SEM for three to four independent experiments.