Table 2.

Co-transfection reporter gene assay

Compound	Vehicle	1 nM Estradiol
1a	0	0
1b	---	---
1c	++	0
1d	---	---
1e	++	0
1f	+++a	0a
1g	0	0
1h	+++	0
1i	+++	0
1j	---	---
1k	+	0
1l	+++a	0a
1m	+	0
2a	+++	0
2b	0	0
2c	+	0
2d	+	0
2e	+a	0a
2f	0	0
2g	0	0
2h	+++	0
2i	0	0
2j	0	0
2k	++	0
2l	+++	0
2m	+	0

(+) Denotes a greater than 50% increase in reporter gene activity at compound concentration <20 μM.

(++) Denotes a greater than 50% increase in reporter gene activity at compound concentration between 20–50 μM.

(+++) Denotes a greater than 50% increase in reporter gene activity at compound concentration >50 μM.

(-) Denotes a greater than 50% decrease in reporter gene activity at compound concentration >50 μM.

(--) Denotes a greater than 50% decrease in reporter gene activity at compound concentration <between 20–50 μM.

(---) Denotes a greater than 50% decrease in reporter gene activity at compound concentration <20 μM.

(0) Denotes a less than 20% change in reporter gene activity. 4 Hydroxytamoxifen was used as an antagonist control.

^aDenotes cell death at concentration greater than 50 μM.

^bDenotes precipitation of compound from solution.