Table 3.

Mammalian two-hybrid competitive inhibition assay

Compound	Vehicle	1 nM Estradiol
1a	–a	0a
1b	---a	---a
1c	0	0
1d	---	---
1e	++	0
1f	0	-
1g	0	-
1h	+++	0
1i	+++	0
1j	---a	---a
1k	+b	+b
1l	+++ab	0ab
1m	0	0
2a	+++ab	0ab
2b	--	--
2c	0	0
2d	0	0
2e	0	-
2f	0a	0a
2g	0	0
2h	+++a	0a
2i	0	0
2j	0	-
2k	+++ab	0ab
2l	0	0
2m	+	+++

(+) Denotes a greater than 50% increase in reporter gene activity at compound concentration <20 μM.

(++) Denotes a greater than 50% increase in reporter gene activity at compound concentration between 20–50 μM.

(+++) Denotes a greater than 50% increase in reporter gene activity at compound concentration >50 μM.

(-) Denotes a greater than 50% decrease in reporter gene activity at compound concentration >50 μM.

(--) Denotes a greater than 50% decrease in reporter gene activity at compound concentration <between 20–50 μM.

(---) Denotes a greater than 50% decrease in reporter gene activity at compound concentration <20 μM.

(0) Denotes a less than 20% change in reporter gene activity. 4 Hydroxytamoxifen was used as an antagonist control.

^aDenotes cell death at concentration greater than 50 μM.

^bDenotes precipitation of compound from solution.