Fig. 1.

ATP release from uninjected and Panx1-expressing oocytes. All colored bars represent data obtained under voltage-clamp conditions; white bars are data from unclamped cells. ATP in the media was measured as luciferase luminescence.(A) ATP release from uninjected oocytes or Panx1-expressing oocytes in the presence of oocyte Ringer solution (OR), 150 mM potassium gluconate (KGlu) solution, or to holding the membrane potential at +40 mV under voltage-clamp conditions. Data are shown as means ± SD. N is indicated above the bars.

(B) Membrane currents and ATP release in uninjected and Panx1-expressing oocytes with the membrane potential held at +40 mV. Blue = uninjected and red = Panx1-injected oocytes.

(C) ATP release from Panx1-expressing oocytes induced by KGlu with and without holding the membrane potential under voltage-clamp conditions at −60, 0, or +40 mV. Voltage-clamp conditions are indicated with blue lines below the graph and blue bars in the graph. Open bars are data from oocytes that were not voltage clamped. The presence of 150 mM KGlu or 100 µM carbenoxolone (CBX) is indicated. Data are shown as means ± SD. N=5 for each measurement.

(D) Membrane currents of oocytes used in experiments shown in C. The voltage traces (top) start with the resting potential followed by the holding potential at +40 mV. Blue current traces: membrane potential stepped from the resting membrane potential to +40 mV. Red current traces: membrane potential stepped from the resting membrane potential to 0 mV in the presence of 150 mM KGlu.