Fig. 2.

K⁺-induced ATP release from Panx1-expressing oocytes. (A) Dose-dependent induction of ATP release from Panx1-expressing oocytes. Panx1-expressing oocytes were exposed for 20 minutes to Ringer solutions with increased K⁺ (replacing Na⁺) and aliquots of the supernatant were collected for ATP measurements with the luciferase assay..

(B) Reversibility of K+-induced ATP release. ATP was measured from the same oocytes before, immediately after the K⁺ stimulus, and 20 or 40 minutes after replacement of the K⁺ stimulus with regular oocyte Ringer solution (OR, wash). (C) ATP release from oocytes expressing Cx46 in the presence of oocyte Ringer solution (OR), or holding the membrane potential at 0 mV under voltage-clamp conditions in the presence or absence of CBX (200 µM). For all panels, data are shown as means ± SD. (N=5 for each measurement)