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. 2014 Jul 25;171(17):4010–4025. doi: 10.1111/bph.12708

Figure 1.

Figure 1

YC-1 induces apoptosis of breast cancer cells. (A) Various breast cancer cell lines were treated with the indicated concentrations of YC-1 for 72 h. Cell viability was determined by the MTT method. (B) MDA-MB-468 and SKBR3 cells were treated with vehicle (DMSO, CTL) or YC-1 (MDA-MB-468: 3 μM for 12 h; SKBR3: 10 μM for 24 h). Changes in cell morphology in response to YC-1 were assessed by phase-contrast microscopy. (C) Cells were treated with the indicated concentrations of YC-1 for 24 h (MDA-MB-468) or 48 h (SKBR3). Cells were lysed and analysed for the indicated proteins by Western blotting. β-actin was used as a loading control. Blots are representative of results from three independent experiments. (D) MDA-MB-468 cells were treated with vehicle (DMSO, as control) or 3 μM YC-1 for 6 h. The cell cycles were analysed by flow cytometry. (E) MDA-MB-468 cells were treated with the indicated concentrations of YC-1 and then the clonogenic assay was conducted. Results are expressed as mean ± SEM of three independent experiments. **P < 0.01, compared with control.