Figure 2.
Betaine supplementation rectifies alcohol-induced adipose tissue dysfunction. C57BL/6 mice were fed a control or alcohol-containing diet with/without betaine supplementation (0.5%, w v−1) for 5 weeks. (A) Epididymal fat pad weights. (B) H&E staining of epididymal adipose tissue shows that betaine supplementation prevents the reduction in adipocyte size induced by chronic alcohol exposure. (C) Plasma glycerol levels. (D) Plasma non-esterified fatty acid levels. Data are means ± SD (n = 8 for PF, BT/PF and BT/AF; n = 6 for AF). Bars with different letters differ significantly (P < 0.05). AF, alcohol-fed; BT, betaine supplementation; PF, pair-fed.