Figure 7.
Betaine supplementation alleviates alcohol-induced inhibition of PP2A and activation of HSL in adipose tissue. C57BL/6 mice were fed a control or alcohol-containing diet with/without betaine supplementation (0.5%, w v−1) for 5 weeks. Total protein extracts from epididymal fat pads were prepared thereafter. Forty micrograms of protein was subjected to Western blot analysis for (methylated) PP2A C subunit and HSL phosphorylation using specific antibodies. (A) PP2A activity in epididymal fat pad. (B) Epididymal adipose tissue PP2A C subunit methylation. (C) Epididymal adipose tissue HSL activity. Data are means ± SD (n = 8 for PF, BT/PF and BT/AF; n = 6 for AF). Bars with different letters differ significantly (P < 0.05). AF, alcohol-fed; BT, betaine supplementation; PF, pair-fed.