SBL test in mice. Time course of the behaviour response to i.t. SP (100 pmol) and PWT2-SP (2.5–250 pmol, panel A); the same results are shown as cumulative nociceptive behaviour displayed over the 30 min period in the panel B. One-way anova followed by the Dunnett's post hoc test revealed a significant effect of PWT2-SP [F(3,31) = 19.87]. Effects of pretreatment with aprepitant (1 mg·kg−1, for 2 h) on nociceptive behaviour induced by PWT2-SP (25 pmol). The time-course is shown in panel C while the same results are shown as cumulative nociceptive behaviour displayed over the 30 min period in the panel D. Data are mean ± SEM of at least eight mice per group. *P < 0.05, significantly different from saline or vehicle; unpaired t-test .(t = 5.33, d.f. = 14).