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CD properties of IscU_wt as purified, Zn2+ depleted, Zn2+ depleted and desalted, and after adding extra Zn2+ to the solution. (A) Comparison of the temperature scans following the wavelength at 222 nm. (B) Comparison of the far-UV spectra showing a lower helical content in the absence of Zn2+ and at low ionic strength.
