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Comparison of the stabilities of IscU_wt with the IscU_D39A, IscU_H105A and IscU_D39A_H105A mutants. The proteins were as purified, that is, EDTA untreated. IscU_wt is indicated in black, IscU_D39A in purple, IscU_H105A in red and IscU_D39A_H105A in green. (A) Comparison of the far-UV spectra of the four proteins recorded at 25°C. (B) Thermal denaturation scans followed by detection of the CD signal at 222 nm.
