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Kinetic Parameters for Nitrocefin Hydrolysis by TEM-1 β-Lactamase Wild Type and Contact Residue Alanine Mutants
| Mutant | Km (μM) | kcat (s−1) | kcat/Km (μM−1 s−1) |
|---|---|---|---|
| WT | 83 ± 16 | 1188 ± 133 | 14 |
| Q99A | 72 ± 17 | 1102 ± 89 | 15 |
| N100A | 71 ± 13 | 1057 ± 145 | 15 |
| L102A | 75 ± 14 | 450 ± 157 | 6 |
| E104A | 64 ± 19 | 992 ± 99 | 16 |
| Y105A | 75 ± 17 | 1126 ± 167 | 15 |
| P107A | 61 ± 16 | 573 ± 189 | 9 |
| E110A | 79 ± 13 | 1175 ± 190 | 15 |
| K111A | 58 ± 13 | 943 ± 178 | 16 |
| M129A | 67 ± 14 | 1004 ± 188 | 15 |
| V216A | 78 ± 19 | 952 ± 113 | 12 |
| M272A | 60 ± 8 | 237 ± 11 | 4 |
