Figure 5.

AFM data. To demonstrate that inhibitors cys₁₅₂₁, cys_2935a, and cys_2935a + cys₁₅₂₁ do not aggregate in isolation, they were grown alone for 3 days at a concentration of 100 µM (i.e., 0:2 molar ratio) under the same conditions as inhibition experiments. In all cases, no fibril formation was observed. Inhibition experiments were undertaken at an Aβ₄₂:inhibitor stoichiometry of 1:2 (row two). In this experiment, the same sample was used as for ThT experiments, in which Aβ₄₂ was grown with inhibitor for 3 days at 37°C. All of the peptides are inhibitory, in particular the combination of cys₂₉₃₅ + cys₁₅₂₁. For reversal experiments, Aβ₄₂ was grown alone for 3 days, after which inhibitor was added at a stoichiometry of 1:2 and followed by a further 3 days incubation to assay for peptide induced reversal of amyloid deposition. Each of the samples were imaged by AFM and panels showing representative images obtained. Also in reversal experiments, it can be clearly observed that for all of inhibitors there is a reduction of fibril, although this is less pronounced than for inhibition experiments. Scale bars are shown.