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Surface biotinylation of plasma membranes of primary human parathyroid cells. Representative immunoblot analysis of surface-biotinylated proteins precipitated with neutravidin-agarose beads and probed with anti-TRPV5 and anti-TRPV6 antibodies, which revealed human TRPV5 and TRPV6 detection. Specificity was confirmed by negative control (nc).
