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. 2014 Aug 28;18(10):1944–1952. doi: 10.1111/jcmm.12372

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© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Confocal images of primary human parathyroid cells maintained at different times of incubation. (A) It shows the expression of TRPV5, TRPV6 and CASR (green), respectively, at 24, 48 and 72 hrs. (B) It shows the co-localization between TRPV5, TRPV6 (green) and CASR (red). The immunofluorescence staining images were detected by a confocal laser scanning microscope (Leica TCS-SP5) by using 40× zoom 1 oil objective with 1.45 NA and a recommended pinhole size of less than 1.0 μm; scale bars: 10 μm.