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. 2014 Sep 12;18(10):1964–1974. doi: 10.1111/jcmm.12367

Figure 1.

Figure 1

Experimental design. Medial and lateral menisci were harvested from young and adult pigs. In study 1, the swine menisci were sectioned into the anterior horns, the bodies and the posterior horns and processed for SAFRANIN-O staining, collagen 1 and 2 immunofluorescent staining, DNA and GAGs quantification, collagen 1 and 2 quantification by Western blot analysis. In study 2, the swine menisci were sectioned into the inner, the intermediate and the external zones to determine the expression levels of collagen 1, collagen 2, Sox9 and aggrecan by real-time PCR.