Skip to main content
. 2014 Jun 9;18(10):1975–1991. doi: 10.1111/jcmm.12331

Figure 5.

Figure 5

Overexpression of GnT-V reverses EMT and suppresses cell migration and invasion in human lung adenocarcinoma H1975 cells. (A) GnT-V overexpression stable H1975 cells were established by using lentiviral gene specific for wild-type GnT-V (wtGnT-V) system and selected by using puromycin. The GnT-V mRNA (bottom) and protein (top) levels in stable cells were determined by RT-PCR and western blot respectively. (B) The cell surface β1,6-GlcNAc branched N-glycans of GnT-V overexpression stable H1975 cells were detected by flow cytometry with L-PHA lectin, the negative control showing the background fluorescence. (C) The cell morphological changes of vehicle and wtGnT-V H1975 cells in response to TGF-β1 (5 ng/ml; 48 hrs) treatment. Photographs were taken of living cells by using a × 20 objective. Insets, representative cell morphology. (D) Overexpression of GnT-V in H1975 cells reduces TGF-β1 (5 ng/ml; 48 hrs)-induced EMT, as determined the protein expression levels of EMT markers (E-cadherin and N-cadherin) by western blot. (E) Overexpression of GnT-V in H1975 cells reduces TGF-β1 (5 ng/ml; 48 hrs)-induced cell migration, as determined by transwell assay. A representative result of three independent experiments (top) and statistic data (bottom) were presented. (F) Overexpression of GnT-V in H1975 cells reduces TGF-β1 (5 ng/ml; 48 hrs)-induced cell invasion, as determined by transwell assay with matrigel. A representative result of three independent experiments (top) and statistic data (bottom) were presented.