Table 1.
Details of primary antibodies
| Primary antibody | Host | Antigen retrieval | Working dilution IHC | Source |
|---|---|---|---|---|
| PDGFRα | Goat | Sodium citrate buffer, pH 6.0 Tris-EDTA buffer, pH 9.0 |
1:100 | Catalogue no. AF-307-NA; R&D Systems, Minneapolis, MN, USA |
| CD34 | Mouse | Sodium citrate buffer, pH 6.0 Tris-EDTA buffer, pH 9.0 |
1:50 | Catalogue no. M7165; Dako, Glostrup, Denmark |
| c-Kit | Rabbit | Tris-EDTA buffer, pH 9.0 | 1:300 | Catalogue no. A4502; Dako |
| αSMA | Mouse | Tris-EDTA buffer, pH 9.0 | 1:500 | Catalogue no. A-2547; Sigma-Aldrich, St. Louis, MO, USA |
| PGP9.5 | Rabbit | Sodium citrate buffer, pH 6.0 | 1:200 | Catalogue no. AB1761; Chemicon-Millipore, Temecula, CA, USA |
| Calreticulin | Chicken | Tris-EDTA buffer, pH 9.0 | 1:200 | Catalogue no. PA1-902A; Thermo Scientific, Runcorn, UK |
| nNOS | Rabbit | Sodium citrate buffer, pH 6.0 Tris-EDTA buffer, pH 9.0 |
1:1500 | Catalogue no. AB5380; Chemicon-Millipore |