Table 1.

Real-time PCR primers

		Oligonucleotide (5′–3′)
Genes (for mRNA)
Oct4	Forward Reverse	5′-GTATTCAGCCAAACGACCATC-3′ 5′-CTGGTTCGCTTTCTCTTTCG-3′
Nanog	Forward Reverse	5′-AATACCTCAGCCTCCAGCAGATG-3′ 5′-TGCGTCACACCATTGCTATTCTTC-3′
Sox2	Forward Reverse	5′-GGGAAATGGGAGGGGTGCAAAAGAGG-3′ 5′-TTGCGTGAGTGTGGATGGGATTGGTG-3′
TERT	Forward Reverse	5′-CGGAAGAGTGTCTGGAGCAA-3′ 5′-GGATGAAGCGGAGTCTGGA-3′
ALP	Forward Reverse	5′-ACTGGTACTCAGACAACGAGAT-3′ 5′-ACGTCAATGTCCCTGATGTTATG-3′
OPN	Forward Reverse	5′-TTGCAGCCTTCTCAGCCAA-3′ 5′-GGAGGCAAAAGCAAATCACTG-3′
FABP4	Forward Reverse	5′-AAAGTCAAGAGCACCATAACC-3′ 5′-TTCAATGCGAACTTCAGTCC-3′
PPARr2	Forward Reverse	5′-GCGATTCCTTCACTGATAC-3′ 5′-TCAAAGGAGTGGGAGTGGTC-3′
βIII-tubulin	Forward Reverse	5′-ACAATTTCATCTTTGGTCAGAGTGG-3′ 5′-TCACACTCCTTCCGCACCAC-3′
NF-L	Forward Reverse	5′-ACAAGCAGAACGCCGACATC-3′ 5′-TCCAAAGCCATCTTCACGTTG-3′
GAPDH	Forward Reverse	5′-CGTGGAAGGACTCATGACCA-3′ 5′-TCCAGGGGTCTTACTCCTTG-3′
miRNAs
Hsa-miR-489	Forward	5′-GTGACATCACATATACGGCAGC-3′
Hsa-miR-17-5p	Forward	5′-CAAAGTGCTTACAGTGCAGGTAG-3′
Hsa-miR-145-5p	Forward	5′-GTCCAGTTTTCCCAGGAATCCCT-3′
Hsa-miR-24-3p	Forward	5′-TGGCTCAGTTCAGCAGGAACAG-3′
Hsa-miR-370	Forward	5′-GCCTGCTGGGGTGGAACCTGGT-3′
Hsa-miR-7-5p	Forward	5′-TGGAAGACTAGTGATTTTGTTGT-3′
Hsa-miR-433	Forward	5′-ATCATGATGGGCTCCTCGGTGT-3′
Genes (for ChIP)
GAPDH	Forward Reverse	5′-TACTAGCGGTTTTACGGGCG-3′ 5′-TCGAACAGGAGGAGCAGAGAGCGA-3′
Sox2	Forward Reverse	5′-AGTTGGACAGGGAGATGGC-3′ 5′-AACCTTCCTTGCTTCCACG-3′
Oct4	Forward Reverse	5′-CTTCCACAGACACCATTGCC-3′ 5′-AGTCCCACCCACTAGCCTTG-3′
Nanog	Forward Reverse	5′-GCCCTATCCAAATCCTATCACTT-3′ 5′-GGTCAGCACAAAATACAGGTCA-3′
TERT	Forward Reverse	5′-GGCTCCCAGTGGATTCGC-3′ 5′-GGAGGCGGAGCTGGAAGG-3′
ALP	Forward Reverse	5′-TGTTGACAGACACAGAGACAGACG-3′ 5′-GTCGGCATCTTCCTTCTGCG-3′
OPN	Forward Reverse	5′-GAGACATATTTCCCCCTACC-3′ 5′-CAGTTGTGAAATGCAGATTGCAC-3′
FABP4	Forward Reverse	5′-GGATGGCCTTGGACTCACTC-3′ 5′-AGAAACACCACAGGAGGCTGA-3′
PPARr2	Forward Reverse	5′-TTAGCAGTTTGGCACAGCTAGG-3′ 5′-TCAGGAAAACTCTGGCTTCTTG-3′