Figure 2.

Co-expression and co-localization of APJ and NTSR1. Determination of cell surface expression of HA-APJ and Myc-NTSR1 by ELISA on intact cells using anti-HA and anti-Myc antibody. Plasmids expressing HA-tagged APJ or Myc-tagged NTSR1 were transfected alone or in combination, as illustrated. Values were presented as mean ± SEM of triplicates (A). pEGFP-APJ (green) and pcDNA3.1-NTSR1 were transfected into HEK293 for 24 hrs, pcDNA3.1-NTSR1 was immunostained with Rhodamine (red) and the cell nuclei were stained with dye-DAPI (blue). Immunofluorescence was measured with a Leica fluorescent microscope (B).