Figure 3.

Assessment of dimerization between APJ and NTSR1 using co-IP. HEK293 cells were mock transfected or transfected with expression vectors of both HA-APJ and Myc-NTSR1 fusion proteins (cotransfected) or with either vector alone. Confirmation of the expression of appropriate constructs was obtained by immunoblotting cell lysates with either anti-Myc or anti-HA antibodies (lower panel). The cell lysates were subsequently immunoprecipitated (IP) with anti-HA antibody and immunoblotted with anti-Myc antibodies (upper panel) to demonstrate the dimerization between APJ and NTSR1.