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. 2014 Aug 28;18(10):2071–2081. doi: 10.1111/jcmm.12404

Figure 4.

Figure 4

HEK293 cells were transfected with a constant amount of pRluc-NTSR1 with increasing amounts of pEGFP-APJ by 1:0.5, 1:1, 1:1.5, 1:2, 1:2.5, 1:3. BRET ratio demonstrated as mBRET (Mbret = BRET × 1000). All the results were expressed as mean ± SEM of at least six independent assays (A). HEK293 cells were transiently cotransfected with pRluc-NTSR1 and pEGFP-APJ (1:1.5), and after 24 hrs upon addition of substrate Coelenterazine-h, cells were treated with or without NT (10−7 M) and/or apelin-13 (10−7 M) for 15 min. and BRET signals were analysed (B). All the results were expressed as mean ± SEM of at least six independent assays. *P < 0.05 versus control; **P < 0.01 versus control.