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. 2014 Aug 28;18(10):2071–2081. doi: 10.1111/jcmm.12404

Figure 7.

Figure 7

Effects of Knockdown APJ on ERK1/2 phosphorylation in HUVECs APJ. shRNA efficiently knocks down APJ expression in HUVEC cells. HUVECs were transfected with APJ shRNA or control shRNA. 48 hrs after transfection, cell lysates were subjected to western blot and incubated with antibodies against APJ. GAPDH was used as the loading control (A). HUVECs were transfected as previous described and treated with apelin-13 or NT at 10−7 M for 15 min. respectively. Cell lysates were subjected to western blot and incubated with antibodies against phosphor-ERK and total ERK. HUVECs without shRNA transfection and agonist stimulation were used as basal control. Quantification of the effects of APJ knockdown on ERK1/2 phosphorylation corrected over total-ERK (C). All the results were expressed as mean ± SEM. *P < 0.05 versus basal group; **P < 0.01 versus basal group; #P < 0.05 versus Control shRNA group.