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Effects of heterodimerization of APJ and NTSR1 on HEK293 cell proliferation. HEK293-APJ cells and HEK293-APJ/NTSR1 cells were treated with apelin-13 (10−7 M) (A). HEK293-NTSR1 cells and HEK293-APJ/NTSR1 cells were treated with NT (10−7 M) for 36 hrs (B). The cell proliferation was measured by CCK assays. All the results were expressed as mean ± SEM.*P < 0.05 versus basal group, **P < 0.01 versus basal group, $P < 0.05 versus Single (apelin-13 or NT) stimulated groups.
