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. 2014 Sep 18;18(10):2125–2134. doi: 10.1111/jcmm.12398

Figure 3.

Figure 3

miR-150 directly targets the c-Myb gene in vitro and in vivo. (A) The putative miR-150-binding site in the c-Myb 3′-UTR. (B) The miR-150-binding site in the c-Myb 3′-UTR was confirmed in the LoVo cells using a cotransfection luciferase assay for the indicated reporters and the miR-150 mimic or for the indicated reporters and the miR-150 inhibitor. (C and E) qRT-PCR and Western blot analysis of in vitro c-Myb levels. (D and F) c-Myb mRNA and protein levels were determined by qRT-PCR and Western blot analysis after injecting the miR-150 mimic, mimic control, miR-150 inhibitor or inhibitor control into established LoVo CRC xenografts. (G) Immunohistochemistry assay for c-Myb immunoreactivity. Bars represent the mean ± SD of three experiments.